Glutaredoxin 1s or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity. Glutaredoxin 1 (GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxin 1s utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the Glutaredoxin 1 system). The amino acid sequence of Glutaredoxin 1 in mouse is highly homologous with that in other mammals (81‑89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.
Mouse Glutaredoxin 1/GLRX1 Antibody
R&D Systems | Catalog # AF3119
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Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Met1-Leu107
Accession # Q9QUH0
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse Glutaredoxin 1/GLRX1 Antibody
Detection of Mouse Glutaredoxin 1 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.Applications for Mouse Glutaredoxin 1/GLRX1 Antibody
Western Blot
Sample: NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Glutaredoxin 1/GLRX1
Alternate Names
Gene Symbol
UniProt
Additional Glutaredoxin 1/GLRX1 Products
Product Documents for Mouse Glutaredoxin 1/GLRX1 Antibody
Certificate of Analysis
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Product Specific Notices for Mouse Glutaredoxin 1/GLRX1 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars