Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 228109
View all IL-16 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-16
Ser1205-Ser1322
Accession # O54824

Specificity

Detects mouse IL-16 in direct ELISAs and Western blots. Shows approximately 50% cross‑reactivity with recombinant human (rh) IL-16 and no cross-reactivity with rmC-10, rhI-309, rhHCC-4, or rhHCC-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse IL-16 Antibody (228109)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑16

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-16

Interleukin 16 (IL-16), also known as lymphocyte chemoattractant factor (LCF), refers to the C-terminal peptide of pro-IL-16 isoform 1, which is a nuclear and cytoplasmic PDZ-containing protein. The C-terminus of pro-IL-16 is cleaved by Caspase-3, resulting in the release of secreted IL-16. Mature IL-16 has been shown to bind the cell surface receptor CD4 and functions as an immunomodulatory cytokine.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Entrez Gene IDs

3603 (Human); 16170 (Mouse)

Gene Symbol

IL16

UniProt

O54824

Additional IL-16 Products

Product Documents for Mouse IL-16 Antibody (228109)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-16 Antibody (228109)

For research use only

Citations for Mouse IL-16 Antibody (228109)

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FAQs

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