Mouse LDLR Quantikine ELISA Kit

R&D Systems | Catalog # MLDLR0

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.064 ng/mL

Assay Range

0.313-20 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse LDLR Quantikine ELISA Kit

The Quantikine Mouse LDL R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LDL R levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse LDL R and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LDL R accurately. Results obtained using natural mouse LDL R showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LDL R.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse LDL R. This kit also recognizes PCSK9/LDL R Complex.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.000 2.133 7.265 1.015 2.136 7.466
Standard Deviation 0.053 0.097 0.355 0.083 0.148 0.428
CV% 5.3 4.5 4.9 8.2 6.9 5.7

Recovery for Mouse LDLR Quantikine ELISA Kit

The recovery of mouse LDL R spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 104 80-114

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse LDL R were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse LDL R ELISA Linearity

Scientific Data Images for Mouse LDLR Quantikine ELISA Kit

Mouse LDL R ELISA Standard Curve

Mouse LDL R ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LDLR

LDLR (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDLR binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface. Lack of LDLR expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Entrez Gene IDs

3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for Mouse LDLR Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse LDLR Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Obesity

Citations for Mouse LDLR Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse LDLR Quantikine ELISA Kit (MLDLR0):

Refer to the product for complete assay procedure.
 
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
  3.   Add 50 µL of Assay Diluent to each well.
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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