Mouse LRP-6 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB2960V Datasheet / COA / SDS

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FAB2960V-100UG

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Mouse LRP-6 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LRP-6 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50 - 60% cross-reactivity with recombinant human (rh) LRP-6 and no cross-reactivity with rhLDL R.

Source

Monoclonal Rat IgG2b Clone # 290913

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse LRP-6
Met1-Pro1368
Accession # O88572

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

Long Name

LDL Receptor-related Protein 6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Alternate Names

ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6