Mouse MFG-E8 Quantikine ELISA Kit

R&D Systems | Catalog # MFGE80

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 µL), Serum (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.02 ng/mL

Assay Range

0.125-8 ng/mL
View all MFG-E8 ELISA Kits »

Product Summary for Mouse MFG-E8 Quantikine ELISA Kit

The Quantikine Mouse MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MFG-E8 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse MFG-E8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MFG-E8.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse MFG-E8

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.506 0.936 3.89 0.509 0.910 3.78
Standard Deviation 0.020 0.042 0.116 0.047 0.071 0.322
CV% 4.0 4.5 3.0 9.2 7.8 8.5

Recovery for Mouse MFG-E8 Quantikine ELISA Kit

The recovery of MFG-E8 spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 99 90-104
Heparin Plasma (n=4) 94 90-98
Serum (n=4) 101 97-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MFG-E8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse MFG-E8 ELISA Linearity

Scientific Data Images for Mouse MFG-E8 Quantikine ELISA Kit

Mouse MFG-E8 ELISA Standard Curve

Mouse MFG-E8 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MFG-E8

MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.

Long Name

Milk Fat Globule EGF Factor 8

Alternate Names

BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1

Entrez Gene IDs

4240 (Human); 17304 (Mouse)

Gene Symbol

MFGE8

Additional MFG-E8 Products

Product Documents for Mouse MFG-E8 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse MFG-E8 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse MFG-E8 Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse MFG-E8 Quantikine ELISA Kit (MFGE80):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of the appropriate Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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