Mouse NAIP Antibody

NAIP-1 (Neuronal Apoptosis Inhibitory Protein 1; also Naip and Birc1) is a 150-160 kDa member of both the NLR/PRR and IAP family of proteins. It is widely, but not ubiquitously expressed, and is found in adipocytes, macrophages, neurons, renal proximal and distal convoluted tubules, and nonproliferative intestinal epithelium. NAIP-1 plays a key regulatory role in caspase activation. It promotes caspase-1 activation, blocks caspase-3 and -7 activation, and by binding neuronal hippocalcin, inhibits caspase-3-independent apoptosis. Mouse NAIP-1 is 1403 amino acids (aa) in length. It contains three consecutive N-terminal BIR (Baculovirus Inhibition of apoptosis Repeat) domains (aa 63-346) and one NACHT region (aa 464-758). The BIR #2 domain is posited to bind both caspase-3 and -7, while the BIR domain #3 likely interacts with hippocalcin. NAIP-1 undergoes proteolysis, generating an N-terminal 100 kDa fragment (likely aa 1- ≈ 869) and a 60 kDa C-terminal fragment (likely aa 870-1403). The C-terminal fragment undergoes further processing to generate a 25 kDa and 35 kDa fragment. There are at least seven numbered NAIP genes in mouse that have arisen by gene duplication. Over the aa range 783-856, they share less than 60% aa sequence identity with NAIP-1. Over the same aa range, mouse NAIP-1 shares 54% aa sequence identity with the only human NAIP gene.