Mouse PCPE-1 Antibody (261845)

Detection of PCPE-1 by Western Blot

The BATokine PCPE-1 contributes to the pathogenesis of HFpEF.Littermate control (Con) or BAT Pcolce-KO mice were fed with a high-fat diet (HFD) and studied during 41–47 weeks of age (middle-aged). BAT adeno-associated virus (AAV) encoding Pcolce was injected in the BAT of C57BL/6NCrSlc mice fed with an HFD and studied at 15 weeks of age (mature adult). BAT-PCOLCE KI mice were also fed with an HFD and studied at 23–25 weeks of age (mature adult). (A, F, and J) Western blot analysis of mouse (A and F) or human (J) PCPE-1 expression in the heart of the indicated mice. (B, G, and K) Echocardiographic findings in mice: E/e’ (marker for diastolic dysfunction), IVSTd (interventricular septum thickness), LVDs (left ventricular systolic dimension), FS (fractional shortening) (n = 14, 10 for B), (n =7, 7 for G), and (n = 8, 7 for K). (C, H, and L) Masson’s trichrome stain of the heart and its quantification (n =5, 5 for C), (n = 5, 6 for H), and (n = 4, 6 for L) in mice. Scale bar: 50 μm. (D, I, and M) ELISA for collagen type I (shown as Collagen 1) testing hearts in mice (n = 9, 10 for D), (n = 5, 6 for I), and (n = 8, 7 for M). (E) Experimental design of the AAV-Pcolce mouse. (N) Schematic demonstrating that ROS/DNA damage/c-Fos/c-Jun signaling upregulates PCPE-1 expression in BAT with aging. PCPE-1 produced from BAT promotes fibrosis in the heart. All data were analyzed by an independent-samples t test. Data information: Representative Masson’s trichrome–stained images from 1 series of observations (C, H, and L), and other data were obtained from 1 representative analytical experiment out of at least 2 independent experiments showing similar results. *P < 0.05, **P < 0.01. Values are presented as mean ± SEM. NS = not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41955016), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PCPE-1 by Western Blot

The BATokine PCPE-1 contributes to the pathogenesis of HFpEF.Littermate control (Con) or BAT Pcolce-KO mice were fed with a high-fat diet (HFD) and studied during 41–47 weeks of age (middle-aged). BAT adeno-associated virus (AAV) encoding Pcolce was injected in the BAT of C57BL/6NCrSlc mice fed with an HFD and studied at 15 weeks of age (mature adult). BAT-PCOLCE KI mice were also fed with an HFD and studied at 23–25 weeks of age (mature adult). (A, F, and J) Western blot analysis of mouse (A and F) or human (J) PCPE-1 expression in the heart of the indicated mice. (B, G, and K) Echocardiographic findings in mice: E/e’ (marker for diastolic dysfunction), IVSTd (interventricular septum thickness), LVDs (left ventricular systolic dimension), FS (fractional shortening) (n = 14, 10 for B), (n =7, 7 for G), and (n = 8, 7 for K). (C, H, and L) Masson’s trichrome stain of the heart and its quantification (n =5, 5 for C), (n = 5, 6 for H), and (n = 4, 6 for L) in mice. Scale bar: 50 μm. (D, I, and M) ELISA for collagen type I (shown as Collagen 1) testing hearts in mice (n = 9, 10 for D), (n = 5, 6 for I), and (n = 8, 7 for M). (E) Experimental design of the AAV-Pcolce mouse. (N) Schematic demonstrating that ROS/DNA damage/c-Fos/c-Jun signaling upregulates PCPE-1 expression in BAT with aging. PCPE-1 produced from BAT promotes fibrosis in the heart. All data were analyzed by an independent-samples t test. Data information: Representative Masson’s trichrome–stained images from 1 series of observations (C, H, and L), and other data were obtained from 1 representative analytical experiment out of at least 2 independent experiments showing similar results. *P < 0.05, **P < 0.01. Values are presented as mean ± SEM. NS = not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41955016), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PCPE-1 by Western Blot

Depletion of PCPE-1 ameliorates HFpEF in obesity and aged mouse models.(A–E) Littermate control (Con) or systemic Pcolce-KO mice fed with a high-fat diet (HFD) from 4 weeks of age or a normal chow diet (NCD) and studied at 41–46 weeks of age (middle-aged). (F–J) Systemic Pcolce-KO mice (74–80 wk) fed with an NCD. (A and F) Western blot analysis of PCPE-1 expression in the heart. (B and G) Echocardiographic findings in the indicated mice: E/e’ (marker for diastolic dysfunction), IVSTd (interventricular septum thickness), LVDs (left ventricular systolic dimension), FS (fractional shortening) (n = 6, 8, 8, 9 for E/e’ and n = 6, 8, 13, 12 for other parameters in B; n = 9, 12 for G). (C and H) Masson’s trichrome stain of the heart. Scale bar: 50 μm. Right panels indicate quantification of relative fibrotic area (C) (n = 7, 5) (H) (n = 4, 5). (D and I) ELISA for cardiac collagen type I (shown as Collagen 1) (n = 10, 9 for D) and (n = 4, 5 for I). (E and J) Treadmill examination test (TMET) showing the running distance (n = 13, 17 for E) and (n = 9, 12 for J). Two-way ANOVA followed by Tukey’s multiple-comparison (B and D) or Dunnett T3 multiple comparison test (C); others: independent-samples t test. Data information: Representative Masson’s trichrome–stained images from 1 series of observations (C and H), and other data were obtained from 1 representative analytical experiment out of at least 2 independent experiments showing similar results. *P < 0.05, **P < 0.01. Values are presented as mean ± SEM. NS = not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41955016), licensed under a CC-BY license. Not internally tested by R&D Systems.