Mouse R-Spondin 1 Antibody (852010)

Name: Mouse R-Spondin 1 Antibody (852010)
Brand: R&D Systems
SKU: MAB3474
Price: 429 USD
Availability: InStock

R&D Systems | Catalog # MAB3474

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 852010

View all R-Spondin 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse R‑Spondin 1
Met1-Gln265
Accession # Q9Z132

Specificity

Detects recombinant mouse (rm) R‑Spondin 1 in ELISA. In ELISA, no cross-reactivity with rmR-Spondin 3 and rhR-Spondin 1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse R-Spondin 1 Antibody (852010)

beta -Catenin Response Induced by R-Spondin 1 and Neutralization by Mouse R‑Spondin 1 Antibody.

Recombinant Mouse R-Spondin 1 (7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R-Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R-Spondin 1 Mono-clonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (1324-WN). The ND₅₀ is typically 0.4-2 µg/mL.

Applications for Mouse R-Spondin 1 Antibody (852010)

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize R‑Spondin 1-induced beta -catenin response in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 500 ng/mL Recombinant Mouse R‑Spondin 1 and 5 ng/mL Recombinant Mouse Wnt-3a.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: R-Spondin 1

RSPO-1 (roof plate-specific spondin; also R-Spondin 1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-Spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.