Mouse/Rat BMP-15/GDF-9B Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5917N Datasheet / COA / SDS

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AF5917N-100UG

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Mouse/Rat BMP-15/GDF-9B Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 a

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse BMP-15/GDF-9B
Gln268-Arg392
Accession # Q9Z0L4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BMP-15/GDF-9B

BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively.

Long Name

Bone Morphogenetic Protein 15

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Alternate Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4