Mouse Reg3B Antibody (518630)

R&D Systems | Catalog # MAB5110

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 518630
View all Reg3B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Reg3B

Glu27-Gly175
Accession # P35230

Specificity

Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Reg3B Antibody (518630)

Detection of Mouse Reg3B antibody by Western Blot.

Detection of Mouse Reg3B by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Reg3B Antibody (518630)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse small intestine tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg3B

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily.  It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons.  Reg3B may play a role in intestinal epithelial cell renewal. It contains one C-type lectin domain.  Mature mouse Reg3B is 91% and 67% aa identical to rat and human Reg3B, respectively.     

Long Name

Regenerating Islet-derived IIIb

Alternate Names

PAP1

Entrez Gene IDs

18489 (Mouse); 24618 (Rat)

Gene Symbol

REG3B

UniProt

P35230

Additional Reg3B Products

Product Documents for Mouse Reg3B Antibody (518630)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Reg3B Antibody (518630)

For research use only

Related Research Areas

C-type Lectin Family
Diabetes

Citations for Mouse Reg3B Antibody (518630)

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