Mouse Sonic Hedgehog/Shh C-Terminus Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB445X Datasheet / COA / SDS

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FAB445X-100UG

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Mouse Sonic Hedgehog/Shh C-Terminus Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse Shh C-terminal peptide in direct ELISAs and Western blots. Does not cross-react in direct ELISAs with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide, or rmShh N-terminal peptide.

Source

Monoclonal Rat IgG2a Clone # 55626

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog
Cys199-Ser437
Accession # Q62226.1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. It is cleaved by autoproteolysis to release an N-terminal signaling peptide that binds the multicomponent receptor complex containing the multi-pass transmembrane proteins Patched and Smoothened. The C-terminal peptide carries the protease domain which acts only in CIS. It also acts on a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N.

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS