Mouse TGF‑ beta RI/ALK‑5 Alexa Fluor™ Plus 488‑conjugated Antibody

Name: Mouse TGF‑ beta RI/ALK‑5 Alexa Fluor™ Plus 488‑conjugated Antibody
Brand: R&D Systems
SKU: AF587AFP488
Price: 589 USD
Availability: InStock

R&D Systems | Catalog # AF587AFP488

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

View all TGF-beta RI/ALK-5 Primary Antibodies »

Product Specifications

Specificity

Detects mouse TGF‑ beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) TGF-beta RI is observed, and less than 2% cross-reactivity with recombinant mouse TGF‑ beta RII and rhTGF‑ beta RIII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TGF‑ beta RI/ALK‑5 Alexa Fluor™ Plus 488‑conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References

Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

BAA05023

Additional TGF-beta RI/ALK-5 Products

Product Documents for Mouse TGF‑ beta RI/ALK‑5 Alexa Fluor™ Plus 488‑conjugated Antibody

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Product Specific Notices for Mouse TGF‑ beta RI/ALK‑5 Alexa Fluor™ Plus 488‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only