Mouse WARP Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4927N Datasheet / COA / SDS

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AF4927N-100UG

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Mouse WARP Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse WARP in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human WARP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse WARP isoform 1
Arg19-Pro415
Accession # Q8R2Z5

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WARP

WARP (von Willebrand factor A [vWFA] domain-related protein) is a 50 kDa glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted mouse WARP is 397 amino acids (aa) in length. It contains a vWFA domain (aa 34‑209), and two fibronectin type III domains (aa 211‑394) that likely bind to the GAG modification of perlecan. Cys369 and Cys393 contribute to intermolecular bond formation. There is one alternate start site at Met213. Mature mouse WARP (aa 19‑415) is 93% and 78% aa identical to rat and human WARP, respectively.

Long Name

von Willebrand factor A Domain-related Protein

Entrez Gene IDs

64856 (Human); 246228 (Mouse); 298683 (Rat)

Alternate Names

DKFZp761O051; von Willebrand factor A domain containing 1; von Willebrand factor A domain-containing protein 1; von Willebrand factor A domain-related protein; VWA1; WARP