Mouse WIF-1 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB135T Datasheet / COA / SDS

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FAB135T-100UG

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Mouse WIF-1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human WIF-1 is observed.

Source

Monoclonal Rat IgG2a Clone # 134714

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse WIF-1
Gly29-Trp379
Accession # Q9WUA1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WIF-1

WIF-1 is a secreted protein of approximately 50 kDa that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Mature mouse WIF-1 shares 95% and 98% amino acid sequence identity with human and rat WIF-1, respectively.

Long Name

Wnt Inhibitory Factor

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Alternate Names

WIF1; WIF-1; WNT inhibitory factor 1