Mouse WIF-1 Antibody (134714)

R&D Systems | Catalog # MAB135

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 134714
View all WIF-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse WIF-1
Gly29-Trp379
Accession # Q9WUA1

Specificity

Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human WIF-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse WIF-1 Antibody (134714)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse WIF‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WIF-1

WIF-1 is a secreted protein of approximately 50 kDa that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Mature mouse WIF-1 shares 95% and 98% amino acid sequence identity with human and rat WIF-1, respectively.

Long Name

Wnt Inhibitory Factor

Alternate Names

WIF1

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Gene Symbol

WIF1

UniProt

Q9WUA1

Additional WIF-1 Products

Product Documents for Mouse WIF-1 Antibody (134714)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse WIF-1 Antibody (134714)

For research use only

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