Mouse Wnt-10b Antibody (254206)

R&D Systems | Catalog # MAB2110

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 254206
View all Wnt-10b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-10b
Arg263-Arg332
Accession # P48614

Specificity

Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -11, or -16 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Wnt-10b Antibody (254206)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Wnt‑10b

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-10b

Wnt-10b, also known as Wnt-12, is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists in two isoforms that are distinguished by the presence or absence of an internal 95 amino acid sequence.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Alternate Names

Wnt-12, Wnt10b

Entrez Gene IDs

7480 (Human); 22410 (Mouse)

Gene Symbol

WNT10B

UniProt

P48614

Additional Wnt-10b Products

Product Documents for Mouse Wnt-10b Antibody (254206)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-10b Antibody (254206)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Citations for Mouse Wnt-10b Antibody (254206)

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FAQs

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