MYO18A Antibody - BSA Free

Novus Biologicals | Catalog # NB100-79839

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MYO18A Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 300 and 350 of human myosin 18A using the numbering given in entry NP_510880.2 (GeneID 399687).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYO18A Antibody - BSA Free

Immunoprecipitation: MYO18A Antibody [NB100-79839]

Immunoprecipitation: MYO18A Antibody [NB100-79839]

Immunoprecipitation: MYO18A Antibody [NB100-79839] - Detection of Human Myo18A/Myosin XVIIIA by Western Blot of Immunoprecipitates. Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myo18A/Myosin XVIIIA antibody NB100-79839 used for IP at 3 mcg/mg lysate. Myo18A/Myosin XVIIIA was also immunoprecipitated by rabbit anti- Myo18A/Myosin XVIIIA antibody NB100-79840, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for MYO18A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg lysate
Application Notes
This antibody has not performed satisfactorily when used for WB of Myo18A/Myosin XVIIIA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myo18A/Myosin XVIIIA.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MYO18A

Myo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE)-rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid-associated lung collectin that mediates innate and adaptive immune responses in the lungs.

Alternate Names

KIAA0216DKFZp686L0243, MAJN, Molecule associated with JAK3 N-terminus, myosin 18A, Myosin containing a PDZ domain, myosin containing PDZ domain, myosin XVIIIA, myosin-XVIIIa, MYSPDZ, SP-A receptor subunit SP-R210 alphaS, SPR210

Entrez Gene IDs

399687 (Human)

Gene Symbol

MYO18A

UniProt

NP_510880.2 (Human)

Additional MYO18A Products

Product Documents for MYO18A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MYO18A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MYO18A Antibody - BSA Free

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Protocols

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