Myosin heavy chain 11 Antibody

Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221]

Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.

Chromatin Immunoprecipitation: Myosin heavy chain 11 Antibody [NBP2-59221] - ChIP assays were performed using ME-1 cells, the antibody against MYH11 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

ELISA: Myosin heavy chain 11 Antibody [NBP2-59221]

ELISA: Myosin heavy chain 11 Antibody [NBP2-59221] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MYH11. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.