N4BP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37688

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all N4BP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 250 to 300 of human NEDD4 Binding Protein 1 using the numbering given in entry NP_694574.3 (GeneID 9683).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N4BP1 Antibody - BSA Free

Western Blot: N4BP1 Antibody [NBP2-37688]

Western Blot: N4BP1 Antibody [NBP2-37688]

Western Blot: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: N4BP1 Antibody [NBP2-37688]

Immunoprecipitation: N4BP1 Antibody [NBP2-37688]

Immunoprecipitation: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for N4BP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: N4BP1

Alternate Names

FLJ31821, KIAA0615, MGC176730, NEDD4 binding protein 1, NEDD4-binding protein 1

Gene Symbol

N4BP1

UniProt

NP_694574.3 (Human)

Additional N4BP1 Products

Product Documents for N4BP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for N4BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for N4BP1 Antibody - BSA Free

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