Nephrin Antibody (G17-H)

Novus Biologicals | Catalog # NBP1-30137

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # G17-H
View all Nephrin Primary Antibodies »

Product Specifications

Immunogen

Peptide derived from C-terminal sequence of first Ig-like domain of human Nephrin. Antibody recognizes the epitope located between Cys53 - Cys101.

Epitope

Cys53 - Cys101

Specificity

This antibody is specific for the C terminus of first Ig-like domain of human nephrin (between residues Cys53-Cys101).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Nephrin Antibody (G17-H)

Western Blot: Nephrin Antibody (G17-H) [NBP1-30137]

Western Blot: Nephrin Antibody (G17-H) [NBP1-30137]

Western Blot: Nephrin Antibody (G17-H) [NBP1-30137] - Western Blot on recombinant protein. Lane 1 - 50ng; lane 2 - 100ng; Lane 3 - 200ng of recombinant mouse nephrin (extracellular domain, Gln 37 - Thr 1049).

Applications for Nephrin Antibody (G17-H)

Application
Recommended Usage

ELISA

1:20000-1:50000

Immunoprecipitation

1:10-1:500

Western Blot

1:2000
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Tween-20. Blocking buffer: 1xTBS; 0.1% Tween-20; 8% nonfat dry milk.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nephrin

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Alternate Names

CNF, NPHS1

Entrez Gene IDs

4868 (Human)

Gene Symbol

NPHS1

UniProt

O60500

Additional Nephrin Products

Product Documents for Nephrin Antibody (G17-H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nephrin Antibody (G17-H)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Immunoglobulin Superfamily CAMs

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