Neuregulin-3/NRG3 Antibody

Novus Biologicals | Catalog # NB100-61670

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Neuregulin-3/NRG3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RNEIQRDSALTK corresponding to C-Terminus according to NP_001010848.2.

Epitope

RNEIQRDSALTK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Neuregulin-3/NRG3 Antibody

Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670]

Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670]

Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670] - Analysis of Neuregulin 3 in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Neuregulin-3/NRG3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human brain (cerebellum) lysates and in mouse and rat brain lysates (calculated MW of 75.0 kDa band according to NP_001010848.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neuregulin-3/NRG3

NRG3, which belongs to the neuregulin family, is a direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. NRG3 does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. The protein exists as an type I membrane protein and as a proteolytically released soluble growth factor form. The membrane-bound form does not appear to be active. NRG3 is highly expressed in most regions of the brain with the exception of corpus callosum, and is expressed at lower level in testis. It is not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. The NRG3 cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing may involve initial intracellular domain dimerization.

Alternate Names

Neuregulin3, NRG3

Entrez Gene IDs

10718 (Human); 18183 (Mouse); 498596 (Rat)

Gene Symbol

NRG3

UniProt

NP_001010848.2 (Human)

Additional Neuregulin-3/NRG3 Products

Product Documents for Neuregulin-3/NRG3 Antibody

Certificate of Analysis

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Product Specific Notices for Neuregulin-3/NRG3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

EGF Family

Citations for Neuregulin-3/NRG3 Antibody

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