NLK Antibody (1C1) - Azide and BSA Free

Novus Biologicals | Catalog # H00051701-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C1

Format

Azide and BSA Free
View all NLK Primary Antibodies »

Product Specifications

Immunogen

NLK (AAH64663, 416 a.a. ~ 515 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Reactivity Notes

Human. Other species not tested.

Specificity

NLK - nemo like kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NLK Antibody (1C1) - Azide and BSA Free

Western Blot: NLK Antibody (1C1) [H00051701-M01]

Western Blot: NLK Antibody (1C1) [H00051701-M01]

Western Blot: NLK Antibody (1C1) [H00051701-M01] - Analysis of NLK antibody HeLa cells. protein load - 40 ug, antibody dilution: 1:1000, in 5% milk. Image from verified customer review.
Sandwich ELISA: NLK Antibody (1C1) [H00051701-M01] - Detection limit for recombinant GST tagged NLK is approximately 0.03ng/ml as a capture antibody.

Applications for NLK Antibody (1C1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00051701-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NLK

Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3

Alternate Names

DKFZp761G1211, EC 2.7.11, EC 2.7.11.24, FLJ21033, LAK1, nemo like kinase, nemo-like kinaseserine/threonine-protein kinase NLK, Protein LAK1

Entrez Gene IDs

51701 (Human)

Gene Symbol

NLK

UniProt

AAH64663 (Human)

Additional NLK Products

Product Documents for NLK Antibody (1C1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NLK Antibody (1C1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NLK Antibody (1C1) - Azide and BSA Free (1)

5 out of 5
1 Customer Rating
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  • NLK Antibody (1C1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 12/13/2021
    HeLa cells. protein load - 40 ug, antibody dilution: 1:1000, in 5% milk.
    Protein load - 40 ug, antibody dilution: 1:1000, in 5% milk.
    NLK Antibody (1C1) - Azide and BSA Free H00051701-M01

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