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Normal Human IgG Control

R&D Systems | Catalog # 1-001-A

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Control

Label

Unconjugated

Antibody Source

Polyclonal Human IgG
View all IgG Isotype Controls »

Product Specifications

Immunogen

Normal human plasma (Donor plasma tested and found negative for HBsAg, HIV-1, HIV-2 and HCV antibodies by FDA approved tests)

Clonality

Polyclonal

Host

Human

Isotype

IgG

Scientific Data Images for Normal Human IgG Control

Detection of Human IgG by Western Blot

Detection of Human IgG by Western Blot

Activated ADSCs trigger the proliferation and invasion of lung cancer cells by regulating MMP2/9 expression and EMT. a The effects of activated ADSCs on lung cancer cell proliferation were evaluated using the CCK-8 assay. Four lung cancer cell lines were cultured in the presence of ADSC-CM or aADSC-CM, and the optical density at 450 nm was analysed. The cancer cells cultured alone in normal growth medium were used as negative controls. Data from three separate experiments are shown. b The number of lung cancer cells that migrated through 8-μm Transwell membrane pores was counted to determine the changes in the invasive capabilities in response to CM from epidural ADSCs or aADSCs. c MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines treated with ADSC-CM or aADSC-CM were examined by western blotting. Lung cancer cells cultured in an untreated medium served as controls. d Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. Lung cancer cell invasion was analysed using a Transwell assay. Lung cancer cells cultured with untreated medium served as negative controls. e Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines were analysed using western blotting. Lung cancer cells cultured with untreated medium served as negative controls. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Normal Human IgG Control

Application
Recommended Usage

Control

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.8 using 1-001-A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 2 mg/mL.

Formulation

10 mM Sodium Phosphate, 0.85% (w/v) Sodium Chloride, pH 7.2 (PBS) with 5% trehalose and 0.05% (w/v) Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

♦ 12 months from date of receipt at -20 to -70 °C

♦ 6 months from date of receipt at 2 to 8 °C

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Normal Human IgG Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Normal Human IgG Control

Contains sodium azide.  Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.

For research use only

Citations for Normal Human IgG Control

Customer Reviews for Normal Human IgG Control (5)

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Customer Images

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  • Normal Human IgG Control
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 02/16/2020
    IgG was used as negative control.
    Normal Human IgG Control 1-001-A
  • Normal Human IgG Control
    Name: Alberto Contreras
    Application: Functional Assay
    Sample Tested: Bladder cancer tissue
    Species: Human
    Verified Customer | Posted 02/04/2020
    UM-UC3 bladder cancer cells were transiently transfected with a Notch-reporter Firefly luciferase plasmid, in addition to an internal Renilla control. IgG was used as a negative stimulation control, whereas Jagged 1 was used to induce a Notch-specific response. Increasing concentrations of the Gamma-secretase inhibitor DAPT resulted in a dose-dependent decrease in Notch signal.
    Normal Human IgG Control 1-001-A
  • Normal Human IgG Control
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/22/2019
    Normal Human IgG Control 1-001-A
  • Normal Human IgG Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MBA-MB-123 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/27/2018
    Used at 10 ug/mL works perfectly as a human Isotype control for flow cytometry. There is no background binding in live cells that do not express Fc receptor. The attached image shows MFI with and without the isotype control indicating identical signal.
    Normal Human IgG Control 1-001-A
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human annulus pulposus cell culture
    Species: Human
    Verified Customer | Posted 01/27/2015
    NP CCR1 flow data
    Normal Human IgG Control 1-001-A

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FAQs for Normal Human IgG Control

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  • Q: What subtype of IgG does Normal Human IgG Control (Catalog # 1-001-A) contain?

    A: Normal Human IgG Control (Catalog # 1-001-A) is produced by purification of IgG from Normal human plasma. It may contain more than one subtype of IgG.

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