Nrf2 Antibody (JE30-14)

Novus Biologicals | Catalog # NBP3-32661

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE30-14 expressed in HEK293
View all Nrf2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Nrf2 aa 550-605. (Uniprot: Q16236)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nrf2 Antibody (JE30-14)

Nrf2 Antibody (JE30-14)

Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661]

Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661] - Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (NBP3-32661) at 1/1,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 2uM MG-132 for 18 hours whole cell lysate
Lane 3: HCT 116 whole cell lysate
Lane 4: HCT 116 treated with 25uM MG-132 for 4 hours whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 68 kDa
Observed band size: 110 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Nrf2 Antibody (JE30-14)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Nrf2 Antibody (JE30-14)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nrf2 Antibody (JE30-14)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Nrf2 Antibody (JE30-14)

There are currently no reviews for this product. Be the first to review Nrf2 Antibody (JE30-14) and earn rewards!

Have you used Nrf2 Antibody (JE30-14)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail