NSD3 Antibody (7B4) - BSA Free

Novus Biologicals | Catalog # NBP1-04324

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 7B4

Format

BSA Free
View all NSD3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human NSD3 (383-660aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for NSD3 Antibody (7B4) - BSA Free

Western Blot: NSD3 Antibody (7B4)BSA Free [NBP1-04324]

Western Blot: NSD3 Antibody (7B4)BSA Free [NBP1-04324]

Western Blot: NSD3 Antibody (7B4) [NBP1-04324] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate

Applications for NSD3 Antibody (7B4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSD3

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.

Alternate Names

EC 2.1.1.43, FLJ20353, histone-lysine N-methyltransferase NSD3, MGC126766, MGC142029, NSD3DKFZp667H044, Nuclear SET domain-containing protein 3, pp14328, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, Wolf-Hirschhorn syndrome candidate 1-like 1, Wolf-Hirschhorn syndrome candidate 1-like protein 1

Gene Symbol

NSD3

UniProt

NP_075447 (Human)

Additional NSD3 Products

Product Documents for NSD3 Antibody (7B4) - BSA Free

Certificate of Analysis

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Product Specific Notices for NSD3 Antibody (7B4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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