Ovalbumin Antibody [HRP]

Novus Biologicals | Catalog # NB120-20546

Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Chicken

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all Ovalbumin Primary Antibodies »

Product Specifications

Immunogen

Ovalbumin [Hen Egg White] (Uniprot: P01012)

Reactivity Notes

Mouse reactivity reported from a verified customer review. Cross reactivity against Ovalbumin from other sources is unknown.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]

Scientific Data Images for Ovalbumin Antibody [HRP]

Western Blot: Ovalbumin Antibody [HRP] [NB120-20546] - Analysis on purified egg white proteins (lysozyme, ovalbumin, ovotransferrin). Image from verified customer review.
Western Blot: Ovalbumin Antibody [HRP] [NB120-20546] - Lane 1: 1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: 1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for ovalbumin. Other band(s): none.
Western Blot: Ovalbumin Antibody [HRP] [NB120-20546] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
Western Blot: Ovalbumin Antibody [HRP] [NB120-20546] - Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody at 1:1,000 overnight at 4C. Secondary antibody: none Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
Ovalbumin Antibody [HRP]

Ovalbumin Antibody [HRP]

Western Blot of Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody. Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody at 1:1,000 overnight at 4C. Secondary antibody: none Block for 30 min at RT. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Applications for Ovalbumin Antibody [HRP]

Application
Recommended Usage

ELISA

1:5000 - 1:25000

Western Blot

1:1000 - 1:5000
Application Notes
This antibody is suitable for western blotting, ELISA, IHC and IP. Researchers should determine optimal titers for applications that are not stated below..

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Reviewed Applications

Read 1 review rated 5 using NB120-20546 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ovalbumin

Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.

Alternate Names

Allergen Gal d 2, CAP 2, CAP2, Cytoplasmic antiproteinase 2, Egg albumin, Gal d II, Hen egg white, PI8, Plakalbumin, Protease inhibitor 8, Serpin B8, serpin peptidase inhibitor clade B member 8, SERPINB14, SERPINB8

Gene Symbol

OVAL

UniProt

P01012 (Chicken)

Additional Ovalbumin Products

Product Documents for Ovalbumin Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ovalbumin Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ovalbumin Antibody [HRP]

Customer Reviews for Ovalbumin Antibody [HRP] (1)

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4 Stars
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Customer Images

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  • Name: Matthew Back
    Application: Western Blot
    Sample Tested: Purified eggwhite proteins (ovalbumin, lysozyme, ovotransferrin)
    Species: Other
    Verified Customer | Posted 10/03/2014
    Western Blot evaluation of 3 related egg proteins, ovalbumin, lysozyme, & ovotransferrin
    Ovalbumin Antibody [HRP] NB120-20546

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