P4HA2 Antibody

Novus Biologicals | Catalog # NB110-40494

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all P4HA2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human Prolyl 4-Hydroxylase Alpha-2 using the numbering given in entry NP_004190.1 (GeneID 8974 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P4HA2 Antibody

Immunoprecipitation: P4HA2 Antibody [NB110-40494]

Immunoprecipitation: P4HA2 Antibody [NB110-40494]

Immunoprecipitation: P4HA2 Antibody [NB110-40494] - Detection of Human P4HA2 on HeLa whole cell lysate using NB110-40494. P4HA2 was also immunoprecipitated by rabbit anti-P4HA2 antibodies NB100-40475 and another P4HA2 antibody.

Applications for P4HA2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NB110-40494 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: P4HA2

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

2-oxoglutarate-4-dioxygenase subunit alpha-2, 4-PH alpha 2, 4-PH alpha-2, alpha polypeptide II, collagen prolyl 4-hydroxylase alpha(II), C-P4Halpha(II), EC 1.14.11.2, Procollagen-proline, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), prolyl 4-hydroxylase subunit alpha-2, prolyl 4-hydroxylase, alpha polypeptide II

Entrez Gene IDs

8974 (Human)

Gene Symbol

P4HA2

UniProt

O15460 (Human)

Additional P4HA2 Products

Product Documents for P4HA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for P4HA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for P4HA2 Antibody (1)

5 out of 5
1 Customer Rating
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4 Stars
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1 Stars
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 Cell Lysate, Sample Amount: 80 ug
    Species: Human
    Verified Customer | Posted 01/26/2010

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