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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 294 and 344 of human Programmed Cell Death 2 using the numbering given in entry NP_002589.2 (GeneID 5134).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDCD2 Antibody
Western Blot: PDCD2 Antibody [NBP2-04042]
Western Blot: PDCD2 Antibody [NBP2-04042] - Detection of human and mouse PDCD2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDCD2 antibody NBP2-04042 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Immunoprecipitation: PDCD2 Antibody [NBP2-04042]
Immunoprecipitation: PDCD2 Antibody [NBP2-04042] - Detection of human PDCD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDCD2 antibody NBP2-04042 used for IP at 3 ug per reaction. PDCD2 was also immunoprecipitated by another rabbit anti-PDCD2 antibody. For blotting immunoprecipitated PDCD2, NBP2-04042 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for PDCD2 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDCD2
Alternate Names
programmed cell death 2, programmed cell death protein 2, RP8MGC12347, Zinc finger protein Rp-8, ZMYND7Zinc finger MYND domain-containing protein 7
Gene Symbol
PDCD2
Additional PDCD2 Products
Product Documents for PDCD2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PDCD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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