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Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Primate, Xenopus
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Synthetic peptide (selective for only PDE3A variant).
Reactivity Notes
Cross-reacts with Human, Mouse, Rat and Dog.
Localization
Cell Membrane
Specificity
Does not cross reacts with PDE4B variants nor with any other PDEs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PDE3A Antibody
Western Blot: PDE3A Antibody [NB300-673]
Western Blot: PDE3A Antibody [NB300-673] - Western blot detection of PDE3A at an antibody dilution of 1:250.Applications for PDE3A Antibody
Application
Recommended Usage
Immunoprecipitation
1:200
Western Blot
1:500
Application Notes
Immunoprecipitation and Western Blot. Detects two protein bands of approximately 60-80 kDa. Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDE3A
Long Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Alternate Names
cGI-PDE, CGI-PDE A
Gene Symbol
PDE3A
Additional PDE3A Products
Product Documents for PDE3A Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PDE3A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunoprecipitation Protocol
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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