Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-54216

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.061 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)
View all Phospholamban ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

精度

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric)

Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric) ELISA: Mouse Phospholamban  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54216]

ELISA: Mouse Phospholamban  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54216]

Kit Contents for Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Gene Symbol

PLN

Additional Phospholamban Products

Product Documents for Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse Phospholamban - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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