Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 450401
View all PKC iota/lambda Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC iota
Ile455-Val596
Accession # P41743

Specificity

Detects endogenous human and mouse PKC iota / lambda in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for PKC iota/lambda Antibody (450401) [CoraFluor™ 1]

PKC iota/lambda Antibody (450401) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for PKC iota/lambda Antibody (450401) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: PKC iota/lambda

Protein Kinase C (PKC) iota/lambda is an atypical member of the PKC family of serine/threonine protein kinases. Human PKC iota is 596 amino acids (aa) in length with a predicted molecular mass of 68 kDa. The rodent ortholog, PKC lambda, shares 98% aa identity to human PKC iota. PKC iota is highly expressed in the brain and lung and is expressed at low levels in many other tissues including pancreatic islets. As an atypical PKC isoform, PKC iota/lambda is calcium-independent and does not require phosphatidylserine, diacylglycerol (DAG), or exogenous phorbol esters for activation. PKC iota/lambda is the catalytic component of the Par3/Par6/atypical PKC complex, a key regulator of the formation and maintenance of epithelial cell polarity, adherens junctions, and tight junctions. PKC iota is also oncogenic and is used as a prognostic marker in multiple cancer types.

Long Name

Protein Kinase C iota

Alternate Names

PRKCI, PRKCL

Additional PKC iota/lambda Products

Product Documents for PKC iota/lambda Antibody (450401) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PKC iota/lambda Antibody (450401) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Intracellular Kinases
PDGF Family

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