CoraFluor™ 1, amine reactive
Tocris Bioscience | Catalog # 7920
Key Product Details
Description
Product Description
CoraFluor™ 1, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1, amine reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, thiol reactive, CoraFluor™ 2, amine reactive, CoraFluor™ 1, Haloalkane, and CoraFluor™ 2, Haloalkane, also available.
Please refer to the protocol for further information on conjugation.
For more information, please see our CoraFluor™ TR-FRET technology flyer .
CoraFluor™ is a trademark of Bio-Techne Corp.
Scientific Data Images for CoraFluor™ 1, amine reactive
CoraFluor™ 1 stability profile.
Stability profiling of CoraFluor™ 1 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).
CoraFluor™ TR-FRET target engagement assay.
High throughput TR-FRET target engagement assay profiling BRD4 inhibitors and Degraders on endogenous BRD4 using a single detection mix consisting of a primary BRD4 antibody, a CoraFluor™ 1 labeled VHH secondary antibody, and a fluorescent JQ1-based tracer (JQ1-FITC Catalog #7722).
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254Optical Data
| λabs | 338 nm |
| Extinction Coefficient (ε) | 23100 M-1cm-1 |
| Quantum Yield (φ) | 0.58 |
| Closest Laser line | 337 nm |
| Reactive Group | PFP ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.157 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications for CoraFluor™ 1, amine reactive
Molecular Weight
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 6 publications citing the usage of this product.
- Payne A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 2022 PMID: 35649410
- Payne Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 2022 PMID: 35298895
- Payne Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 2021 PMID: 34675420
Product Documents for CoraFluor™ 1, amine reactive
Product Specific Notices for CoraFluor™ 1, amine reactive
For research use only
Citations for CoraFluor™ 1, amine reactive
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