Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-52761

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.112 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

精度

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric)

Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric) ELISA: Human PMCA2  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-52761]

ELISA: Human PMCA2  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-52761]

Kit Contents for Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PMCA2

The protein encoded by the PMCA2 gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Alternate Names

ATPase, Ca++ transporting, plasma membrane 2, plasma membrane calcium-transporting ATPase 2, PMCA2, PMCA2a, PMCA2i

Gene Symbol

ATP2B2

Additional PMCA2 Products

Product Documents for Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human PMCA2 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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