PMVK/phosphomevalonate kinase Antibody (2B8) - Azide and BSA Free

Novus Biologicals | Catalog # H00010654-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B8

Format

Azide and BSA Free
View all PMVK/phosphomevalonate kinase Primary Antibodies »

Product Specifications

Immunogen

PMVK (AAH07694, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPLGGAPRLVLLFSGKRKSGKDFVTEALQSRLGADVCAVLRLSGPLKEQYAQEHGLNFQRLLDTSTYKEAFRKDMIRWGEEKRQADPGFFCRKIVEGISQPIWLVSDTRRVSDIQWFREAYGAVTQTVRVVALEQSRQQRGWVFTPGVDDAESECGLDNFGDFDWVIENHGVEQRLEEQLENLIEFIRSRL

Specificity

Reacts with phosphomevalonate kinase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PMVK/phosphomevalonate kinase Antibody (2B8) - Azide and BSA Free

Western Blot: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07]

Western Blot: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07]

Western Blot: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07] - Analysis of PMVK expression in transfected 293T cell line by PMVK monoclonal antibody (M07), clone 2B8. Lane 1: PMVK transfected lysatE (22 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07]

Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07]

Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07] - Analysis of PMVK transfected lysate using anti-PMVK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PMVK rabbit polyclonal antibody.
Sandwich ELISA: PMVK/phosphomevalonate kinase Antibody (2B8) [H00010654-M07] - Detection limit for recombinant GST tagged PMVK is 3 ng/ml as a capture antibody.

Applications for PMVK/phosphomevalonate kinase Antibody (2B8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PMVK/phosphomevalonate kinase

PMVK (EC 2.7.4.2) is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate as the fifth reaction of the cholesterol biosynthetic pathway.[supplied by OMIM]

Alternate Names

hPMK, HUMPMKI, phosphomevalonate kinase, PMKA, PMKase, PMKEC 2.7.4.2, PMKI

Entrez Gene IDs

10654 (Human)

Gene Symbol

PMVK

UniProt

AAH07694 (Human)

Additional PMVK/phosphomevalonate kinase Products

Product Documents for PMVK/phosphomevalonate kinase Antibody (2B8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PMVK/phosphomevalonate kinase Antibody (2B8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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