Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Human, Porcine

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Western Blot, ELISA Capture, ELISA Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 77724
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant porcine IL‑1 beta /IL-1F2
Ala115-Pro267
Accession # P26889.

Specificity

Detects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL‑1 beta and recombinant mouse (rm) IL‑1 beta is observed and no cross-reactivity with rhIL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Porcine IL‑1 beta /IL‑1F2 Antibody

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine IL‑1 beta /IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine IL‑1 beta /IL‑1F2 Antibody.

Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL.

Applications for Porcine IL‑1 beta /IL‑1F2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 4-15 µg/mL in the presence of 75 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2.

Porcine IL-1 beta /IL-1F2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF681)
  • Standard: Recombinant Porcine IL-1 beta/IL-1F2 Protein (Catalog # 681-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using MAB6811 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.

References

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Huether, M. et al. (1993) Gene 129:285.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P26889.

Additional IL-1 beta/IL-1F2 Products

Product Documents for Porcine IL‑1 beta /IL‑1F2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Porcine IL‑1 beta /IL‑1F2 Antibody

For research use only

Citations for Porcine IL‑1 beta /IL‑1F2 Antibody

Customer Reviews for Porcine IL‑1 beta /IL‑1F2 Antibody (4)

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Showing  1 - 4 的 4 reviews Showing All
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  • Porcine IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Porcine
    Verified Customer | Posted 11/17/2018
    Porcine IL‑1 beta /IL‑1F2 Antibody MAB6811
  • Porcine IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Porcine
    Verified Customer | Posted 04/25/2018
    Porcine IL‑1 beta /IL‑1F2 Antibody MAB6811
  • Porcine IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Porcine
    Verified Customer | Posted 11/07/2017
    We used MAB6811 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF6911 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples.
    Porcine IL‑1 beta /IL‑1F2 Antibody MAB6811
  • Porcine IL-1 beta /IL-1F2 Antibody
    Name: Revathi Shanmugasundaram
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: swine
    Verified Customer | Posted 09/07/2017
    Porcine IL‑1 beta /IL‑1F2 Antibody MAB6811

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