POU5F1P1 Antibody

Novus Biologicals | Catalog # NBP1-52164

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all POU5F1P1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TPGYGSPHFTALYS corresponding to internal region (near C-Terminus) according to NP_001153014.1.

Epitope

C-TPGYGSPHFTALYS

Marker

Embryonic Stem Cell Marker

Specificity

This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for POU5F1P1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 28 kDa band in human heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6 kDa band according to NP_001153014.1. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POU5F1P1

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is nucleus protein, with a predicted molecular weight 39 kD. The poly6318 antibody recognizes human Oct4 and has been shown to be useful for immunofluorescent staining.

Alternate Names

OCT4-PG1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like, OTF3Coctamer binding protein 3_like sequence, OTF3P1, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU class 5 homeobox 1B, POU domain class 5, transcription factor 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain transcription factor OCT4-pg1, POU domain, class 5, transcription factor 1 pseudogene 1, POU5F1P1, POU5F1P4, POU5FLC20, POU5FLC8, putative POU domain, class 5, transcription factor 1B

Gene Symbol

POU5F1B

UniProt

NP_001153014.1 (Human)

Additional POU5F1P1 Products

Product Documents for POU5F1P1 Antibody

Certificate of Analysis

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Product Specific Notices for POU5F1P1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POU5F1P1 Antibody

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