PPP2R5E Antibody

Novus Biologicals | Catalog # NB100-845

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PPP2R5E Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LKRGLRRDGIIPT corresponding to C-Terminus according to NP_006237.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PPP2R5E Antibody

Western Blot: PPP2R5E Antibody [NB100-845]

Western Blot: PPP2R5E Antibody [NB100-845]

Western Blot: PPP2R5E Antibody [NB100-845] - Staining (0.1ug/ml) of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PPP2R5E Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx. 55 kDa band and 40 kDa band bands observed in mouse brain extracts. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36): 22081-9). An anonymous customer has reported usage in human.

Reviewed Applications

Read 1 review rated 3 using NB100-845 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R5E

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily.

Long Name

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

Alternate Names

epsilon isoform of regulatory subunit B56, protein phosphatase 2A, PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, PP2A, B subunit, B' epsilon, PP2A, B subunit, B56 epsilon, PP2A, B subunit, PR61 epsilon, PP2A, B subunit, R5 epsilon, protein phosphatase 2, regulatory subunit B (B56), epsilon isoform, protein phosphatase 2, regulatory subunit B', epsilon isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilonisoform

Entrez Gene IDs

5529 (Human); 26932 (Mouse)

Gene Symbol

PPP2R5E

UniProt

Q16537 (Human)

Additional PPP2R5E Products

Product Documents for PPP2R5E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PPP2R5E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPP2R5E Antibody

Customer Reviews for PPP2R5E Antibody (1)

3 out of 5
1 Customer Rating
5 Stars
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3 Stars
100%
2 Stars
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1 Stars
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cell, Sample Amount: 10ug
    Species: Human
    Verified Customer | Posted 02/16/2009

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