Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
R&D Systems | Catalog # PRD1845
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
Accession Number
Applications
ProDots™ proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots™ proteins are neatly bundled into the shape of a ball. In this form ProDots™ proteins can be rolled directly into cell culture media. ProDots™ proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots™ proteins can be conveniently stored at 4 ºC where they will remain stable for 6 months. The accessible storage and high stability of ProDots™ proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is typically 0.1-0.4 μg/mL.
Scientific Data Images for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein Bioactivity
ProDots®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 0.1-0.4 μg/mL.
ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa.Formulation, Preparation, and Storage
PRD1845
| Reconstitution | For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thawcycles. • 6 months from date of receipt at room temperatureas supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions afterreconstitution. |
Calculators
Background: Sonic Hedgehog/Shh
Alternate Names
Gene Symbol
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
Product Specific Notices for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
726244.0
For research use only
Customer Reviews for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
There are currently no reviews for this product. Be the first to review ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein and earn rewards!
Have you used ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
