Progesterone/OHP ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1896

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Saliva (25 uL)

Sensitivity

n/a

Assay Range

10 to 1000 pg/mL
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Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Description

17-OH-Progesterone ELISA Kit is an enzyme immunoassay for the quantitative measurement of active free 17-OH-Progesterone in saliva.

精度

Intra-Assay Precision (Precision within an assay) CV% 5.23%

Inter-Assay Precision (Precision between assays) CV% 5.33%

Recovery for Progesterone/OHP ELISA Kit (Colorimetric)

Recovery

100.98%

Scientific Data Images for Progesterone/OHP ELISA Kit (Colorimetric)

ELISA: Progesterone/OHP ELISA Kit (Colorimetric) [KA1896]

ELISA: Progesterone/OHP ELISA Kit (Colorimetric) [KA1896]

ELISA: Progesterone/OHP ELISA Kit (Colorimetric) [KA1896] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Progesterone/OHP ELISA Kit (Colorimetric)

  • Control 1 & Control 2: contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.For control values and ranges please refer to vial label or QC-Datasheet. Ready to use.
  • Cortisol Standard A, 0 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Cortisol Standard B, 10 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Cortisol Standard C, 50 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Cortisol Standard D, 250 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Cortisol Standard E, 500 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Cortisol Standard F, 1000 pg/ml, contain 0.03% Proclin 300, 0.015% BND and 0.010% MIT as preservative.
  • Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal)
  • Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal) 96 well (12x8 strips) Enzyme conjugate: 17a-OHP conjugated to horseradish peroxidase, contain 0.03 % Proclin 300, 0.015% BND and 0.010% MIT as preservative. Ready to use.
  • Stopping Solution: containing 0.5 M H2SO4. Avoid contact with the stop solution. It may cause skin irritations and burns.
  • Substrate Solution: Tetramethylbenzidine (TMB)
  • Wash Solution (40X concentrated)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Progesterone/OHP

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain.

Additional Progesterone/OHP Products

Product Documents for Progesterone/OHP ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Progesterone/OHP ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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