Bovine Prokineticin R1/PROKR1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-55727

Novus Biologicals

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

0.23 ng/mL (example only; lot dependent)

Assay Range

0.937 - 60 ng/mL (example only; lot dependent)
View all Prokineticin R1/PROKR1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Bovine

Description

Assay Length: 4.5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Bovine Prokineticin R1/PROKR1 ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Prokineticin R1/PROKR1

PKR (protein kinase, activatable by RNA) is a double-stranded (ds) RNA-activated protein kinase that plays a key role in the innate immunity response to viral infection in higher eukaryotes (reviewed in Langland et al, 2006). PKR contains an N-terminal dsRNA-binding domain that consists of consensus dsRNA-binding motifs and a C-terminal kinase domain containing conserved motifs for protein kinase activity. DsRNA is a well characterized danger signal that the cell uses recognize the presence of viral infection. PKR binds to dsRNA molecules during viral infection and triggers antiviral innate defense mechanisms including the induction of type I interferons and downregulation of gene expression. One of the most well characterized substrates of activated PKR is the eukaryotic tranlation initiation facter, eIF2. PKR phosphorylates eIF2 during virus infection, which ultimately leads to an inhibition in protein synthesis and block in viral replication. The key role played by PKR in innate responses to viral infections is underscored by the large number of DNA and RNA viruses, whose hosts range from insects to humans, that code for PKR inhibitors. PKR is also known as PRKR, EIF2AK1, and EIF2AK2. Recognizes PKR; human PKR is 942 amino acid protein.

Long Name

Prokineticin Receptor 1

Alternate Names

GPR73, GPR73a, PKR1, ProkineticinR1, PROKR1, ZAQ

Gene Symbol

PROKR1

Additional Prokineticin R1/PROKR1 Products

Product Documents for Bovine Prokineticin R1/PROKR1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Bovine Prokineticin R1/PROKR1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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