Prostaglandin E2 (PGE2) is a prostanoid fatty acid derivative of arachidonic acid whose synthesis can be blocked by corticosteroids or by nonsteroidal anti-inflammatory drugs (NSAIDs). It is produced by a wide variety of tissues and is elevated in many bodily fluids during inflammation. PGE2 interacts with EP1, 2, 3, and 4 receptors to induce a complex range of effects. In the kidney, this includes stimulation of renin release and both promotion and inhibition of salt and water absorption. PGE2 can promote either smooth muscle relaxation or contraction. PGE2 is important in normal joint physiology and is a principal mediator of the inflammatory response to tissue damage. PGE2 sensitizes peripheral nociceptive nerves and exaggerates inflammatory and neuropathic pain by direct inhibition of spinal cord glycinergic neurotransmission. PGE2 also plays a role in hippocampal synaptic plasticity and febrile responses. PGE2 activity is linked to the synthesis and release of several hormones and the fetal hypothalamus-pituitary-adrenal axis. PGE2 also stimulates tumor cell proliferation and differentiation as well as tumor-associated neovascularization.
Prostaglandin E2 Parameter Assay Kit
R&D Systems | Catalog # KGE004B
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Prostaglandin E2 Parameter Assay Kit
Product Specifications
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
精度
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 234 | 895 | 1456 | 253 | 835 | 1331 |
| Standard Deviation | 21 | 45 | 89 | 33 | 82 | 120 |
| CV% | 9.0 | 5.0 | 6.1 | 12.9 | 9.9 | 9.0 |
Recovery for Prostaglandin E2 Parameter Assay Kit
The recovery of PGE2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 84-106 |
| EDTA Plasma (n=4) | 93 | 82-108 |
| Heparin Plasma (n=4) | 94 | 81-111 |
| Serum (n=4) | 97 | 86-114 |
| Urine (n=4) | 99 | 81-111 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of PGE2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Prostaglandin E2 Parameter Assay Kit
Multi-species Prostaglandin E2/PGE2 ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Prostaglandin E2/PGE2
Alternate Names
Additional Prostaglandin E2/PGE2 Products
Product Documents for Prostaglandin E2 Parameter Assay Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Prostaglandin E2 Parameter Assay Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Prostaglandin E2 Parameter Assay Kit
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Customer Reviews for Prostaglandin E2 Parameter Assay Kit (12)
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Sample Tested: Tissue Culture MediaVerified Customer | Posted 03/05/2024Standard curve was not accurate and ELISA produced variable results amongst sample replicates.Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 02/13/2024Unfortunately unable to produce a usable standard curve after multiple attempts. There was high variability between replicates as well as potential issues with binding based on OD readings even after repeating experiment and making sure to follow manufacturer's protocol exactly. Kit was easy to use however and instructions were very clear.
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. -
Sample Tested: SerumVerified Customer | Posted 02/05/2022This kit is really great and easy to use!
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Sample Tested: Cell Culture SamplesVerified Customer | Posted 01/16/2022It performed really good and repeatable.
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Sample Tested: Cell Culture MediaVerified Customer | Posted 05/26/2021I used RAW 264.7 cells and found great variability within technical replicates. The image shows the variability (across row) of the standards and I was also not able to get a usable standard curve.
Bio-Techne ResponseThank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Sample Tested: Cell culture supernatantVerified Customer | Posted 04/03/2021High variability between replicates. Perhaps the most common issue with the kit is the requirement to complete the assay with a specific microplate shaker- which is how the assay was developed and QC tested, this is a horizontal orbital microplate shaker (0.12 inches or 3mm orbit) set at 500 ± 50 rpm.
Bio-Techne ResponseThank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Sample Tested: Cell culture supernatantVerified Customer | Posted 11/04/2020I had trouble replicating the standard curve
Bio-Techne ResponseThank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 11/07/2019
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Sample Tested: Cell Culture MediaVerified Customer | Posted 11/03/2017Very easy to use, reliable results
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Sample Tested: Cell Culture MediaVerified Customer | Posted 07/17/2017
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Sample Tested: Adipose tissueVerified Customer | Posted 05/02/2017
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Sample Tested: Mesenchymal stem cellsVerified Customer | Posted 06/21/2016
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FAQs for Prostaglandin E2 Parameter Assay Kit
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Q: How should the indomethacin be prepared, prior to being added to the serum or plasma collection tubes?
A: First, dissolve indomethacin (Tocris cat # 1708) in ethanol to 10 mg/mL. Then, dilute indomethacin solution 1:50 in 1X PBS (to 0.2 mg/mL). Finally, inject 0.2 mg/mL indomethacin solution into vacutainer tube immediately following blood draw, to a final concentration of 10 μg/mL.
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Q: What is the species reactivity of the Prostaglandin E2 Parameter Assay Kit?
A: The Parameter PGE2 Immunoassay is a competitive enzyme immunoassay designed to measure PGE2 level in various sample types, and the kit can be used for multiple species.
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Q: How should the indomethacin be prepared, prior to being added to the serum or plasma collection tubes?
A: First, dissolve indomethacin (Tocris cat # 1708) in ethanol to 10 mg/mL. Then, dilute indomethacin solution 1:50 in 1X PBS (to 0.2 mg/mL). Finally, inject 0.2 mg/mL indomethacin solution into vacutainer tube immediately following blood draw, to a final concentration of 10 μg/mL.
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Q: What is the species reactivity of the Prostaglandin E2 Parameter Assay Kit?
A: The Parameter PGE2 Immunoassay is a competitive enzyme immunoassay designed to measure PGE2 level in various sample types, and the kit can be used for multiple species.