Protein Kinase A regulatory subunit I alpha Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93193

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein Kinase A regulatory subunit I alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Protein Kinase A regulatory subunit I alpha (NP_002725.1). MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Kinase A regulatory subunit I alpha Antibody - BSA Free

Western Blot: Protein Kinase A regulatory subunit I alpha AntibodyAzide and BSA Free [NBP2-93193]

Western Blot: Protein Kinase A regulatory subunit I alpha AntibodyAzide and BSA Free [NBP2-93193]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Western blot analysis of extracts from normal (control) and Protein Kinase A regulatory subunit I alpha knockout (KO) 293T cells, using Protein Kinase A regulatory subunit I alpha antibody (NBP2-93193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western Blot: Protein Kinase A regulatory subunit I alpha AntibodyAzide and BSA Free [NBP2-93193]

Western Blot: Protein Kinase A regulatory subunit I alpha AntibodyAzide and BSA Free [NBP2-93193]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Analysis of extracts of various cell lines, using Protein Kinase A regulatory subunit I alpha at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] -

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] - Western blot analysis of lysates from Mouse testis using Protein Kinase A regulatory subunit I alpha Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] -

Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] - Immunofluorescence analysis of NIH/3T3 cells using Protein Kinase A regulatory subunit I alpha Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] -

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody - Azide and BSA Free [NBP2-93193] - Western blot analysis of lysates from wild type (WT) and Protein Kinase A regulatory subunit I alpha knockout (KO) 293T cells, using [KO Validated] Protein Kinase A regulatory subunit I alpha Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Applications for Protein Kinase A regulatory subunit I alpha Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Gene Symbol

PRKAR1A

Additional Protein Kinase A regulatory subunit I alpha Products

Product Documents for Protein Kinase A regulatory subunit I alpha Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Protein Kinase A regulatory subunit I alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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