PrPSc Antibody (V21-V)

Novus Biologicals | Catalog # NBP1-71678

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # V21-V
View all PrPSc Primary Antibodies »

Product Specifications

Immunogen

Peptide derived from the middle of the second alpha-helical domain of human PrPc protein. Antibody recognizes the epitope located between Val161 - His177.

Epitope

Val161 - His177

Specificity

Antibody recognizes mostly PrPSC, untreated with proteinase-K however, PrPC is slightly recognized as well.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for PrPSc Antibody (V21-V)

Western Blot: PrPSc Antibody (V21-V) [NBP1-71678]

Western Blot: PrPSc Antibody (V21-V) [NBP1-71678]

Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 ug of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells, transfected with mouse prion protein bearing 3F4 epitope (C); lane D corresponds to 30 ng recombinant MoPrP.
Western Blot: PrPSc Antibody (V21-V) [NBP1-71678]

Western Blot: PrPSc Antibody (V21-V) [NBP1-71678]

Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and untransfected HEK 293 cells (C). 200 ug of total protein loaded per well.

Applications for PrPSc Antibody (V21-V)

Application
Recommended Usage

ELISA

1:20000-1:50000

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PrPSc

Prion diseases are transmissible neurodegenerative disorders which affect a range of mammalian species. In humans they can be inherited and sporadic as well as acquired by exposure to human prions. Prions appear to be composed principally of a conformational isomer of host-encoded prion protein and propagate by recruitment of cellular prion protein (1). The function of the cellular prion protein (PrP) is still poorly understood. It has been proposed that one unprecedented role for PrP is against Bax-mediated neuronal apoptosis. It has been shown that PrP potently inhibits Bax-induced cell death in human primary neurons (2). An impaired synaptic inhibition may be involved in the epileptiform activity seen in Creutzfeldt-Jakob and other neurodegenerative diseases and it is believed that loss of function of PrP may contribute to the early synaptic loss and neuronal degeneration seen in these diseases (3).

Alternate Names

Prion Protein Scrapie

Gene Symbol

PRNP

Additional PrPSc Products

Product Documents for PrPSc Antibody (V21-V)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PrPSc Antibody (V21-V)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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