Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-52332

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

11.6 pg/mL (example only; lot dependent)

Assay Range

31.25 - 2000 pg/mL (example only; lot dependent)
View all PSP ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PSP

Parotid secretory protein (PSP; also short palate, lung, nasal epithelium carcinoma-associated protein 2 or SPLUNC2 and C20orf70) is a 36 - 40 kDa secreted glycoprotein and member of the Bactericidal permeability-increasing (BPI)/Lipopolysaccharide-binding protein (LBP)/PLUNC superfamily and PLUNC family of proteins (1). Human PSP is synthesized as a 249 amino acid (aa) precursor that contains an 18 aa signal sequence and a 231 aa mature chain. The mature chain contains one BPI/LBS/PLUNC domain (aa 62 - 220) and two potential sites for N-linked glycosylation. Mature human PSP is 31% and 26% aa identical to mature mouse and rat PSP, respectively. Human PSP is expressed in the parotid and submandibular glands in ductal epithelial cells and acinar cells (1 - 2). PSP has been shown to exhibit bacteristatic and bactericidal effects on Pseudomonas aerginosa (1). In addition, PSP-derived peptides inhibit the binding of endotoxin to LBP and inhibit the endotoxin-stimulated secretion of tumor necrosis factor alpha from macrophages (1, 3). These findings suggest that PSP peptides can serve as templates for the design of novel anti-inflammatory peptides (3). One study showed that peptide GL-13 induced bacterial matting, suggesting passive agglutination of bacteria (4). GL-13 was shown to agglutinate Gram negative bacteria P. aerginosa and Aggregatibacter (Actinobacillus) actinomycetemcomitans, Gram positive Streptococcus gordonii and uncoated sheep erythrocytes (4). The agglutination leads to increased clearance by host phagocytic cells.

Long Name

Parotid Secretory Protein

Alternate Names

BPIFA2, C20orf70, SPLUNC2

Gene Symbol

BPIFA2

Additional PSP Products

Product Documents for Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Inflammatory Mediators

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