Q: Does this antibody also recognize Cdc28-PSTAIR from budding yeast (S. cerevisiae) lysate?

A: NB120-10345 is predicted to have cross reactivity with a wide range of species based on the sequence homology, although we haven't tested it specifically in yeast, but I did a blast search and it has 100% homology with S. cerevisiae cdk1 so most likely it should work in yeast as well.

PSTAIR Antibody (PSTAIR)

Novus Biologicals | Catalog # NB120-10345

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Avian, Plant, Primate, Xenopus

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # PSTAIR

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Product Specifications

Immunogen

Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA.

Epitope

Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).

Reactivity Notes

Cross-reacts with human, monkey, mouse, Japanese quail, fish (goldfish, carp, eel, starfish, and amago salmon), Xenopus, cliliates, and plants (lily and onion). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PSTAIR (PSTAIR)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for PSTAIR Antibody (PSTAIR)

Western Blot: PSTAIR Antibody (PSTAIR) [NB120-10345]

Western Blot: PSTAIR Antibody (PSTAIR) [NB120-10345] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-PSTAIR Clone:PSTAIR. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SK-N-SH 4. SH-SY5Y 5. U87 6. COS7 7. PC12 8. Neuro-2a 9. Arabidopsis

Applications for PSTAIR Antibody (PSTAIR)

Application

Recommended Usage

ELISA

1:100-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Application Notes

ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2; when it is complexed with cyclin B.WB: Use at a dilution of 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Raw ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSTAIR

The cell cycle is controlled in part by cyclin-dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.

Alternate Names

EGVPSTAIREISLLKE, EGVPSTAIREISLLKE sequence, PSTAIR sequence

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Product Documents for PSTAIR Antibody (PSTAIR)

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Product Specific Notices for PSTAIR Antibody (PSTAIR)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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