PTEN [p Ser29, p Thr32] Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77932

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chimpanzee, Rhesus Macaque

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTEN Primary Antibodies »

Product Specifications

Immunogen

This affinity purified PTEN [p Ser29, p Thr32] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 223-237 of human PTEN protein. (Uniprot: O43460)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Modification

p Ser29, p Thr32

Specificity

This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the pS29 and pT32 residues. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human PTEN pS29/pT32 protein and the antibody is specific for the phosphorylated form of the proteins. Approximately 3% reactivity is observed against the non-phosphorylated form of the immunizing peptide. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the [p Ser29] and [p Thr 32] residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PTEN [p Ser29, p Thr32] Antibody - BSA Free

Western Blot: PTEN [p Ser29, p Thr32] Antibody [NBP1-77932]

Western Blot: PTEN [p Ser29, p Thr32] Antibody [NBP1-77932]

Western Blot: PTENP1 [p Ser29, p Thr32] Antibody [NBP1-77932] - Analysis of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng. Blocking: BlockOut Buffer. Primary Antibody: Anti-IL-1a. Secondary Antibody: Goat anti-Rabbit HRP.

Applications for PTEN [p Ser29, p Thr32] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:80000

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PTEN

This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Gene Symbol

PTEN

UniProt

O43460 (Human)

Additional PTEN Products

Product Documents for PTEN [p Ser29, p Thr32] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTEN [p Ser29, p Thr32] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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