Rat PTGER2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00461

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

23.44 pg/mL

Assay Range

39.06 - 2500 pg/mL
View all PTGER2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat PTGER2 in samples. No significant cross-reactivity or interference between Rat PTGER2 and analogues was observed.

精度

Intra-Assay Precision (Precision within an assay) 8.96%

Inter-Assay Precision (Precision between assays) 8.86%

Recovery for Rat PTGER2 ELISA Kit (Colorimetric)

Recovery

88 - 111%

Linearity

Rat PTGER2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat PTGER2 ELISA Kit (Colorimetric)

Rat PTGER2 ELISA Kit (Colorimetric)

ELISA: Rat PTGER2 ELISA Kit (Colorimetric) [NBP3-00461] -

ELISA: Rat PTGER2 ELISA Kit (Colorimetric) [NBP3-00461] - Standard Curve Reference

Kit Contents for Rat PTGER2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PTGER2

Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism.Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors,designated EP1, EP2, EP3 and EP4. The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney. EP1,which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers. EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase. EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion. EP3 is expressed in renal vessels, thick ascending limb and collecting duct. EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. In uterus, EP3 induces the contraction of uterine smooth muscles.EP4 is expressed in glomerulus and collecting duct. It couples to Gs proteins,which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release.

Long Name

Prostaglandin E2 Receptor 2, Subtype EP2

Alternate Names

EP2

Gene Symbol

PTGER2

Additional PTGER2 Products

Product Documents for Rat PTGER2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat PTGER2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat PTGER2 ELISA Kit (Colorimetric)

Customer Reviews for Rat PTGER2 ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Rat PTGER2 ELISA Kit (Colorimetric) and earn rewards!

Have you used Rat PTGER2 ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits