PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00005802-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1H6

Format

Azide and BSA Free
View all PTP sigma/PTPRS Primary Antibodies »

Product Specifications

Immunogen

PTPRS (AAH29496, 31 a.a. ~ 128 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EPPRFIKEPKDQIGVSGGVASFVCQATGDPKPRVTWNKKGKKVNSQRFETIEFDESAGAVLRIQPLRTPRDENVYECVAQNSVGEITVHAKLTVLRGP

Localization

Cell Membrane; Single-pass type I membrane protein.

Specificity

PTPRS - protein tyrosine phosphatase, receptor type, S

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

Immunoprecipitation: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01]

Immunoprecipitation: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01]

PTP-sigma-PTPRS-Antibody-1H6-Immunoprecipitation-H00005802-M01-img0003.jpg
Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01]

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01]

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - PTPRS monoclonal antibody (M01), clone 1H6 Western Blot analysis of PTPRS expression in HeLa ( Cat # L013V1 ).
Sandwich ELISA: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Detection limit for recombinant GST tagged PTPRS is 0.03 ng/ml as a capture antibody.
PTP sigma/PTPRS Antibody (1H6)

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] -

Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Characterization of purified monoclonal antibodies targeting RPTP sigma.COS7 cells were transfected with either human RPTP sigma (hRPTP sigma ) or mouse RPTP sigma (HA-mRPTPs) & 24 hrs post-transfection, western blot (A) & immunoprecipitation (B) analysis was performed using the indicated purified monoclonal antibodies. Antibody 1H6 reacted with both forms was used as positive control. Actin was the loading controls. C: Differentiated SH-SY5Y cells were immunostained using the purified 1.3H12 or 4.5H5 monoclonal antibodies for RPTP sigma (green), Dapi for the nucleus (blue) & Phalloidin for actin (red). IgG was used as negative control. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0178489), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTP sigma/PTPRS

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.

Long Name

Protein Tyrosine Phosphatase Receptor-type Sigma

Alternate Names

PTPR sigma, PTPRS

Entrez Gene IDs

5802 (Human)

Gene Symbol

PTPRS

OMIM

601576 (Human)

UniProt

AAH29496 (Human)

Additional PTP sigma/PTPRS Products

Product Documents for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for PTP sigma/PTPRS Antibody (1H6) - Azide and BSA Free

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