Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81219

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
View all Pyruvate Dehydrogenase E1-alpha subunit ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse Pyruvate Dehydrogenase E1-alpha subunit in samples. No significant cross-reactivity or interference between Mouse Pyruvate Dehydrogenase E1-alpha subunit and analogues was observed.

精度

Intra-Assay Precision (Precision within an assay) CV% 4.22%

Inter-Assay Precision (Precision between assays) CV% 4.74%

Recovery

Recovery

90-110%

Linearity

Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

Scientific Data Images

Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

ELISA: Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81219] -

ELISA: Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81219] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pyruvate Dehydrogenase E1-alpha subunit

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Alternate Names

EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Gene Symbol

PDHA1

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customer Reviews

There are currently no reviews for this product. Be the first to review Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) and earn rewards!

Have you used Mouse Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits