Rab1A Antibody (4G10) - BSA Free

Novus Biologicals | Catalog # NBP3-11043

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G10

Format

BSA Free
View all Rab1A Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant Rab1A protein (NP_004152.1)

Localization

Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Specificity

Detects ~23 kDa. Cross-reacts with RAB1B and RAB1C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rab1A Antibody (4G10) - BSA Free

Western Blot: Rab1A Antibody (4G10) [NBP3-11043]

Western Blot: Rab1A Antibody (4G10) [NBP3-11043]

Western Blot: Rab1A Antibody (4G10) [NBP3-11043] - Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of Rab1A protein using Mouse Anti-Rab1A Monoclonal Antibody, Clone 4G10 (NBP3-11043). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10 ug (A549, MEF); 20 ug (Mouse Brain). Primary Antibody: Mouse Anti-Rab1A Monoclonal Antibody (NBP3-11043) at 1:1000.
Immunoprecipitation: Rab1A Antibody (4G10) [NBP3-11043]

Immunoprecipitation: Rab1A Antibody (4G10) [NBP3-11043]

Immunoprecipitation: Rab1A Antibody (4G10) [NBP3-11043] - Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10. Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody.
Rab1A Antibody (4G10)

Western Blot Shows Human Rab1A Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and Rab1A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Rab1A Antibody (Catalog # NBP3-11043) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for Rab1A (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Rab1A Antibody (4G10)

Knockout Validation of Rab1A Antibody in HAP1 and Rab1A Knockout HAP1 Cells in Immunocytochemistry/Immunofluorescence.

HAP1 WT and Rab1A KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rab1A antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/100. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Rab1A Antibody (4G10) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Rab1A

The small GTPase RAB1 controls vesicle traffic from the endoplasmic reticulum (ER) to the Golgi apparatus. Rab1 belongs to the Ras superfamily of GTPases that cycle between inactive GDP-bound and active GTP-bound forms (Allan et al., 2000 [PubMed 10903204]).[supplied by OMIM]

Long Name

Ras-related protein Rab-1A

Alternate Names

RAB1

Gene Symbol

RAB1A

Additional Rab1A Products

Product Documents for Rab1A Antibody (4G10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Rab1A Antibody (4G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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